Antioxidant Capacity and Anti-inflammatory Effect of the Ethyl Acetate Fraction of Dried Persimmon (Diospyros kaki Thumb.) on THP-1 Human Acute Monocytic Leukemia Cell Line

Abstract

Antioxidant capacity and anti-inflammatory effect of ethyl acetate (EtOAc) fraction of phenolic extract from dried persimmon (Diospyros kaki Thumb.) were investigated on THP-1 human acute monocytic leukemia cells. Phenolic extract of dried persimmon was sequentially fractionized using five different solvents, hexane, chloroform, EtOAc, butanol, and water, among which EtOAc fraction showed the highest antioxidant capacity. Cytotoxicity of THP-1 cells pretreated with the EtOAc fraction and curcumin was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Curcumin and the EtOAc fraction showed respective cytotoxicities at 30 and 100 mu g/mL. Expression level of proinflammatory tumor necrosis factor-alpha (TNF-alpha) was quantified by enzyme-linked immunosorbent assay. TNF-alpha production was significantly inhibited by curcumin and the EtOAc fraction at 20 and 50 mu g/mL, respectively. These results showed that phenolic compounds of dried persimmon alleviated the augmentation of TNF-alpha level induced by lipopolysaccharide in THP-1 cells, suggesting that dietary uptake of dried persimmon may be beneficial to promote anti-inflammation.