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Optimization and evaluation of heat-shock condition for spore enumeration being used in thermal-process verification: Differential responses of spores and vegetative cells of Clostridium sporogenes to heat shock

Authors
Byun, Bo YoungLiu, YanhongTang, JumingKang, Dong-HyunCho, Hong-YonHwang, Han-JoonMah, Jae-Hyung
Issue Date
6월-2011
Publisher
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
Keywords
Clostridium sporogenes; spore; vegetative cell; enumeration; heat shock
Citation
FOOD SCIENCE AND BIOTECHNOLOGY, v.20, no.3, pp.751 - 757
Indexed
SCIE
SCOPUS
KCI
Journal Title
FOOD SCIENCE AND BIOTECHNOLOGY
Volume
20
Number
3
Start Page
751
End Page
757
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/112312
DOI
10.1007/s10068-011-0105-7
ISSN
1226-7708
Abstract
To evaluate a heat-shock condition for the enumeration of Clostridium sporogenes spores, a surrogate for C. botulinum spores, we examined the heat tolerance of C. sporogenes spores and vegetative cells exposed to a heat shock at 90A degrees C. From the D values of the spores determined in the temperature range of 113-121A degrees C, z value (+/- SD) and D-90A degrees C value were estimated to be 10.16 +/- 0.90A degrees C and 1,071.52 min, respectively, and the inactivation rates were predicted to be only approximately 2% at 90A degrees C for up to 10 min. Meanwhile, the viable count of spores was significantly higher when activated under a heat-shock condition of 90A degrees C for over 9 min than those activated for shorter time periods. The heat tolerance of vegetative cells was extremely low, showing a D-90A degrees C value (+/- SD) of 0.21 +/- 0.01 min. Finally, 3 different heat-shock conditions were compared: 70A degrees C for 30 min, 80A degrees C for 20 min, and 90A degrees C for 10 min, and the experimental comparative data showed no significant differences in viable spore counts. Consequently, these results support that the heat-shock treatment at 90A degrees C for 10 min is suitable to activate spores and to inactivate vegetative cells of C. sporogenes.
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