Occurrence and mechanisms of amikacin resistance and its association with beta-lactamases in Pseudomonas aeruginosa: a Korean nationwide study
- Authors
- Kim, Ja-Young; Park, Yeon-Joon; Kwon, Hi Jeong; Han, Kyungja; Kang, Moon Won; Woo, Gun-Jo
- Issue Date
- 9월-2008
- Publisher
- OXFORD UNIV PRESS
- Keywords
- aminoglycoside-modifying enzymes; aph(3 ' )-VI; beta-lactamases; integrons
- Citation
- JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, v.62, no.3, pp.479 - 483
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
- Volume
- 62
- Number
- 3
- Start Page
- 479
- End Page
- 483
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/122785
- DOI
- 10.1093/jac/dkn244
- ISSN
- 0305-7453
- Abstract
- Objectives: We investigated the occurrence and mechanism of amikacin resistance and its association with various beta-lactamase genes in Pseudomonas aeruginosa isolates. Methods: Of the total 250 consecutive, non-duplicated isolates of P. aeruginosa, 55 isolates showed amikacin resistance. PCR amplification of genes for aminoglycoside (AG)-modifying enzymes [aac(3)-I, aac(3)-II/VI, aac(3)-III/IV, aac(6')-I, aac(6')-II, ant(2 '')-I, ant(4')-II and aph(3')-VI], 16S rRNA methylases (rmtA, rmtB, rmtC and armA) and class 1 integrons was performed. In addition, we analysed the association of AG resistance genes with various beta-lactamase genes. Results and conclusions: In Korea, the amikacin resistance rate in P. aeruginosa was high (22%), and it varied among provinces (3.8% to 40%). Four types of AG-modifying enzyme genes [aph(3')-VI, ant(2 '')-I, aac(6')-I and aac(3)-II/VI] were found in 48 isolates. Thirty-six strains harboured two or more types of enzymes, of which a combination of aph(3')-VI and ant(2 '')-I was the most frequent (24/36 isolates, 66.7%). None harboured aac(3)-I, aac(3)-III/IV, aac(6')-II, ant(4')-II, rmtA, rmtB, rmtC or armA. Forty-two isolates co-harboured beta-lactamase genes (mostly bla(OXA-10)). A class 1 integron was detected in all but one, and all the ant(2 '')-I and 26/29 bla(OXA-10) were found in it. In contrast, aph(3')-VI was not found to be associated with the class 1 integron. Considering the possibility of co-selection and dissemination, constant monitoring of resistance evolution is necessary.
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