Macrophage-stimulating activity of exo-biopolymer from cultured rice bran with Monascus pilosus
- Authors
- Yu, KW; Kim, YS; Shin, KS; Kim, JM; Suh, HJ
- Issue Date
- Jul-2005
- Publisher
- HUMANA PRESS INC
- Keywords
- macrophage-stimulating activity; Monascus pilosus; rice bran; submerged culture
- Citation
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.126, no.1, pp.35 - 48
- Indexed
- SCIE
SCOPUS
- Journal Title
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
- Volume
- 126
- Number
- 1
- Start Page
- 35
- End Page
- 48
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/123230
- DOI
- 10.1007/s12010-005-0004-6
- ISSN
- 0273-2289
- Abstract
- To find a new use of rice bran, five fungi were examined for the production of exo-biopolymer with macrophage-stimulating activity from rice bran. Among the exo-biopolymers produced from the cultures, Monascus pilosus had the most potent macrophage stimulating activity in a liquid culture rather than in a solid culture. In order to improve the yield of exo-biopolymer with macrophage-stimulating activity, a suitable medium for exo-biopolymer was tested in submerged culture of M. pilosus. The highest amount of exo-biopolymer (13.9 mg/mL) was obtained in a medium containing rice bran as an only carbon source followed by media with additional maltose and sucrose (13.8 and 13.7 mg/mL, respectively). The addition of peptone resulted in the production of high amount of exo-biopolymer (15.1 mg/mL), meanwhile the addition of ammonium chloride resulted in 264.0 mu g/mL of glucosamine content. Among eight different kinds of inorganic salts tested, potassium phosphate (0.1%) was the most effective inorganic salt for the mycelial growth and exo-biopolymer production. Therefore the optimal medium composition was as follows (g/L): 20 g of rice bran, 5 g of peptone, and 1 g of KH2PO4. The optimal culture pH and time for mycelial growth and exo-biopolymer production was pH 5.0 and 25 degrees C, respectively. The maximum exo-biopolymer (20.1 mg/mL) was observed at the fourth day of cultivation. Exo-biopolymer, a crude polysaccharide fraction, mainly contained neutral sugar (81.8%) with considerable amounts of uronic acid (18.2%). Component sugar analysis showed that the active fraction consisted mainly of arabinose, galactose, glucose, which was digested from starch of rice bran during cultivation, and uronic acid (molar ratio; 0.8:1.0:0.7:0.8).
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