Anti-depressant effects of ethanol extract from Cannabis sativa (hemp) seed in chlorpromazine-induced Drosophila melanogaster depression model
- Authors
- Ahn, Yejin; Han, Sung Hee; Kim, Min Guk; Hong, Ki-Bae; Kim, Woo Jung; Suh, Hyung Joo; Jo, Kyungae
- Issue Date
- 1-Jan-2021
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Behavioural pattern; cannabinoid; neurotransmitter
- Citation
- PHARMACEUTICAL BIOLOGY, v.59, no.1, pp.998 - 1007
- Indexed
- SCIE
SCOPUS
- Journal Title
- PHARMACEUTICAL BIOLOGY
- Volume
- 59
- Number
- 1
- Start Page
- 998
- End Page
- 1007
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/137816
- DOI
- 10.1080/13880209.2021.1949356
- ISSN
- 1388-0209
- Abstract
- Context Depression is a severe mental illness caused by a deficiency of dopamine and serotonin. Cannabis sativa L. (Cannabaceae) has long been used to treat pain, nausea, and depression. Objective This study investigates the anti-depressant effects of C. sativa (hemp) seed ethanol extract (HE) in chlorpromazine (CPZ)-induced Drosophila melanogaster depression model. Materials and methods The normal group was untreated, and the control group was treated with CPZ (0.1% of media) for 7 days. The experimental groups were treated with a single HE treatment (0.5, 1.0, and 1.5% of media) and a mixture of 0.1% CPZ and HE for 7 days. The locomotor activity, behavioural patterns, depression-related gene expression, and neurotransmitters level of flies were investigated. Results The behavioural patterns of individual flies were significantly reduced with 0.1% CPZ treatment. In contrast, combination treatment of 1.5% HE and 0.1% CPZ significantly increased subjective daytime activity (p < 0.001) and behavioural factors (p < 0.001). These results correlate with increased transcript levels of dopamine (p < 0.001) and serotonin (p < 0.05) receptors and concentration of dopamine (p < 0.05), levodopa (p < 0.001), 5-HTP (p < 0.05), and serotonin (p < 0.001) compared to those in the control group. Discussion and conclusions Collectively, HE administration alleviates depression-like symptoms by modulating the circadian rhythm-related behaviours, transcript levels of neurotransmitter receptors, and neurotransmitter levels in the CPZ-induced Drosophila model. However, additional research is needed to investigate the role of HE administration in behavioural patterns, reduction of the neurotransmitter, and signalling pathways of depression in a vertebrate model system.
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Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
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