Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo
- Authors
- Hong, Ki Bae; Kim, Jae Hwan; Kwon, Hyuk Kon; Han, Sung Hee; Park, Yooheon; Suh, Hyung Joo
- Issue Date
- Apr-2016
- Publisher
- FACULTY FOOD TECHNOLOGY BIOTECHNOLOGY
- Keywords
- high-purity galactooligosaccharides; bifidobacteria; genes encoding GLP-1 and PYY peptides
- Citation
- FOOD TECHNOLOGY AND BIOTECHNOLOGY, v.54, no.2, pp.156 - 163
- Indexed
- SCIE
SCOPUS
- Journal Title
- FOOD TECHNOLOGY AND BIOTECHNOLOGY
- Volume
- 54
- Number
- 2
- Start Page
- 156
- End Page
- 163
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/89045
- DOI
- 10.17113/ftb.54.02.16.4292
- ISSN
- 1330-9862
- Abstract
- Galactooligosaccharides (GOS) are an important class of dietary prebiotics that exert beneficial effects on intestinal microbiota and gut barrier function. In this study, high-purity GOS (HP-GOS) were investigated in vitro and in vivo and confirmed as prebiotic ingredients in rat diet. HP-GOS were successfully produced using a two-step process, enzymatic hydrolysis and fermentation by yeast. They were found to serve as a good substrate and carbon source for supporting the growth of probiotic bacteria more effectively than other commercial GOS. Following administration of 1 % (by mass) of HP-GOS to rats, the growth of Bifidobacterium bifidum and B. longum in the gut increased most rapidly up to 12 h, and thereafter the increase was slow. Therefore, 1 % HP-GOS was found to be acceptable for the growth of probiotic bacteria. Groups of animals that were orally administered HP-GOS and bifidobacteria during the study, and the group administered HP-GOS during the 2nd (days 13-15) and 4th (days 28-30) period of the study had significantly (p<0.05) higher numbers of bifidobacteria in faeces than groups receiving a single dose of bifidobacteria. HP-GOS affected the expression of genes encoding glucagon-like peptide-1 (GLP-1) and peptide YY (PYY). There was a significant upregulation of GLP-1 and PYY mRNA with HP-GOS and bifidobacteria intake. We propose that the prebiotic properties of HP-GOS are potentially valuable for the production of functional foods for human consumption.
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Collections - College of Health Sciences > School of Biosystems and Biomedical Sciences > 1. Journal Articles
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