Chemical properties and immuno-stimulating activities of crude polysaccharides from enzyme digests of tea leaves
- Authors
- Park, H.-R.; Suh, H.J.; Yu, K.-W.; Kim, T.Y.; Shin, K.-S.
- Issue Date
- 2015
- Publisher
- Korean Society of Food Science and Nutrition
- Keywords
- Complement; Cytokine; Green tea; Macrophage; Polysaccharide
- Citation
- Journal of the Korean Society of Food Science and Nutrition, v.44, no.5, pp.664 - 672
- Indexed
- SCOPUS
KCI
- Journal Title
- Journal of the Korean Society of Food Science and Nutrition
- Volume
- 44
- Number
- 5
- Start Page
- 664
- End Page
- 672
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/95948
- DOI
- 10.3746/jkfn.2015.44.5.664
- ISSN
- 1226-3311
- Abstract
- In order to develop new immuno-stimulating ingredients from mature leaves of green tea, crude polysaccharides were isolated from pectinase digests of tea leaves (green tea enzyme digestion, GTE-0), after which their immuno-stimulating activities and chemical properties were examined. GTE-0 mainly contained neutral sugars (54.9%) such as glucose (14.2%), arabinose (12.2%), rhamnose (11.1%), and galacturonic acid (45.1%), which are characteristic of pectic polysaccharides. The anti-complementary activity of GTE-0 was similar to that of polysaccharide K (used as positive control). Number of morphologically activated macrophages was significantly increased in the GTE-0-treated group. GTE-0 significantly augmented H2O2 and reactive oxygen species production by murine peritoneal macrophage cells in a dose-dependent manner, whereas production of nitric oxide showed the highest activity at a dose of 100 μg/mL among all tested concentrations. Murine peritoneal macrophages stimulated with GTE-0 showed enhanced production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factors-α in a dose-dependent manner. Further, GTE-0 induced higher phagocytic activity in a dose-dependent manner. In ex vivo assay for cytolytic activity of murine peritoneal macrophages, GTE-0-treated group showed significantly higher activity compared to the untreated group at an effector-to-target cell ratio of 20. The above results lead us to conclude that polysaccharides from leaves of green tea have a potent immuno-stimulating effect on murine peritoneal macrophage cells. © 2015, J Korean Soc Food Sci Nutr. All right reserved.
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