Rapid enzymatic assay of biogenic amines in Doenjang and Gochujang using amine oxidase

Abstract

In order to conduct fast detection of biogenic amines in doenjang and gochujang, an enzymatic assay using an amine oxidase from Arthrobacter aurescens (AMAO) was conducted. AMAO showed high activity for monoamines and histamine. A standard curve for histamine determined by AMAO was linear up to 50 mg/kg. Analysis of 16 samples revealed that biogenic amine contents determined by the enzymatic methods showed a good agreement with the sum of 3 monoamines and histamine by HPLC (y=1.039x and R (2)=0.992). The limit of detection of the enzymatic method was 0.22 mg/kg. The average values of the percent of coefficient validation for the doenjang and gochujang were 2.7 and 9.8%, respectively. These results suggest that the enzymatic assay using AMAO can be considered as a reliable method for the quantitative determination of the sum of the monoamines and histamine in doenjang and gochujang.