Grape Seed Extract Regulates Androgen Receptor-Mediated Transcription in Prostate Cancer Cells Through Potent Anti-Histone Acetyltransferase Activity
- Authors
- Park, Si Yong; Lee, Yoo-Hyun; Choi, Kyung-Chul; Seong, Ah-Reum; Choi, Hyo-Kyoung; Lee, Ok-Hee; Hwang, Han-Joon; Yoon, Ho-Geun
- Issue Date
- 1월-2011
- Publisher
- MARY ANN LIEBERT, INC
- Keywords
- androgen receptor; grape seed extract; histone acetyltransferase; prostate cancer
- Citation
- JOURNAL OF MEDICINAL FOOD, v.14, no.1-2, pp.9 - 16
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MEDICINAL FOOD
- Volume
- 14
- Number
- 1-2
- Start Page
- 9
- End Page
- 16
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/113371
- DOI
- 10.1089/jmf.2010.1264
- ISSN
- 1096-620X
- Abstract
- Histone acetylation, which is regulated by histone acetyltransferases (HATs) and deacetylases, is an epigenetic mechanism that influences eukaryotic transcription. Significant changes in histone acetylation are associated with cancer; therefore, manipulating the acetylation status of key gene targets is likely crucial for effective cancer therapy. Grape seed extract (GSE) has a known protective effect against prostate cancer. Here, we showed that GSE significantly inhibited HAT activity by 30-80% in vitro (P < .05). Furthermore, we demonstrated significant repression of androgen receptor (AR)-mediated transcription by GSE in prostate cancer cells by measuring luciferase activity using a pGL3-PSA construct bearing the AR element in the human prostate cancer cell line LNCaP (P < .05). GSE treatment also decreased the mRNA level of the AR-regulated genes PSA and NKX 3.1. Finally, GSE inhibited growth of LNCaP cells. These results indicate that GSE potently inhibits HAT, leading to decreased AR-mediated transcription and cancer cell growth, and implicate GSE as a novel candidate for therapeutic activity against prostate cancer.
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- Appears in
Collections - College of Science and Technology > Department of Food and Biotechnology > 1. Journal Articles
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