Characterizations of Environmental Factors in Conjugated Linoleic Acid Production by Mixed Rumen Bacteria
- Authors
- Choi, Nag-Jin; Park, Hui Gyu; Kim, Jun Ho; Hwang, Han-Joon; Kwon, Ki Han; Yoon, Jin A.; Kwon, Eung Gi; Chang, Jongsoo; Hwang, In Ho; Kim, Young Jun
- Issue Date
- 14-10월-2009
- Publisher
- AMER CHEMICAL SOC
- Keywords
- CLA; rumen bacteria; biohydrogenation; glucose; linoleic acid
- Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.57, no.19, pp.9263 - 9267
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Volume
- 57
- Number
- 19
- Start Page
- 9263
- End Page
- 9267
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/119114
- DOI
- 10.1021/jf9021465
- ISSN
- 0021-8561
- Abstract
- Conjugated linoleic acid (CLA) production by rumen bacteria is closely related to biohydrogenation of linoleic acid (LA) and affected by various environmental factors. Ruminal biohydrogenation and isomerization were characterized in view of incubation conditions using a mixed culture of microorganisms obtained from surgically prepared cows. Free-floating bacteria (FFB) produced more CLA than particle-attached bacteria (PAB). Some major factors affecting the ruminal environment. such as diet, concentrations of fat substrates, incubation time, pre-incubation, and the presence of glucose in the medium were found to be important determinants for the ruminal production of CLA and in a close relationship with biohydrogenation. The mixed bacterial culture, which was pre-exposed to LA, produced more CLA than an unexposed control in a medium containing 30% rumen fluid. The rate of conversion of fat substrate (LA) to hydrogenated products (trans-C18:1, C18:0) was negatively correlated with the initial LA concentration. Overall, the present study showed that CLA accumulation can be increased by modification of diet-induced environmental conditions, which affect changes in ruminal microflora.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Science and Technology > Department of Food and Biotechnology > 1. Journal Articles
- Graduate School > Department of Food and Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.