Development of a Virus Elution and Concentration Procedure for Detecting Norovirus in Oysters
- Authors
- Ha, Sookhee; Woo, Gun-Jo; Hwang, In-Gyun; Choi, Weon Sang
- Issue Date
- 10월-2009
- Publisher
- KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
- Keywords
- elution; concentration; norovirus; poliovirus; oyster
- Citation
- FOOD SCIENCE AND BIOTECHNOLOGY, v.18, no.5, pp.1150 - 1154
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- FOOD SCIENCE AND BIOTECHNOLOGY
- Volume
- 18
- Number
- 5
- Start Page
- 1150
- End Page
- 1154
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/119205
- ISSN
- 1226-7708
- Abstract
- Low levels of virus contamination and naturally occurring reverse transcription-polymerase chain reaction (RTPCR) inhibitors restrain virus detection in oysters. A rapid and efficient oyster-processing procedure that can be used for sensitive virus detection in oysters was developed. Poliovirus type I Sabin strain was used to evaluate the efficacy of virus recovery. The procedure included (a) acid-adsorption and elution with buffers (0.25 M glycine-0.14 M NaCl, pH 7.5; 0.25 M threonine-0.14 M NaCl, pH 7.5); (b) polyethylene glycol (PEG) precipitation; (c) resuspension in Tween 80/Tris solution and chloroform extraction; (d) the second PEG precipitation; (e) viral RNA extraction with TRIzol and isopropanol precipitation; and (f) RT-PCR combined with semi-nested PCR. The overall recovery of elution/concentration was 19.5% with poliovirus. The whole procedure usually takes 19 hr. The overall detection sensitivity was 4 RT-PCR units of genogroup I norovirus (NoV) and 6.4 RT-PCR units of genogroup II Nov/25 g of oysters initially seeded. The virus-detecting method developed in this study should facilitate the detection of low levels of NoV in oysters.
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Collections - College of Life Sciences and Biotechnology > Division of Food Bioscience and Technology > 1. Journal Articles
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