Preparation of Nanoemulsions of Vitamin A and C by Microfluidization: Efficacy on the Expression Pattern of Milk-Specific Proteins in MAC-T Cells
- Authors
- Kim, Tae-Il; Kim, Tae-Gyun; Lim, Dong-Hyun; Kim, Sang-Bum; Park, Seong-Min; Hur, Tai-Young; Ki, Kwang-Seok; Kwon, Eung-Gi; Vijayakumar, Mayakrishnan; Kim, Young-Jun
- Issue Date
- 2-7월-2019
- Publisher
- MDPI
- Keywords
- nanoemulsion; microfluidization; vitamins; casein
- Citation
- MOLECULES, v.24, no.14
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULES
- Volume
- 24
- Number
- 14
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/64164
- DOI
- 10.3390/molecules24142566
- ISSN
- 1420-3049
- Abstract
- In this study, we prepared stabilized vitamin A and C nanoemulsions, and investigated their efficacy on milk-specific proteins in bovine mammary epithelial cells (MAC-T). Emulsions of vitamin A (vit-A) and C (vit-C) were prepared using Lipoid S 75 and microfluidization. The particle size and polydispersity index (PDI) of nanoemulsified vit-A and vit-C were studied. The cytotoxic effect of nanoemulsion-free and nanoemulsified vit-A and vit-C was determined by an MTT assay. In addition, the efficacy of nanoemulsified vit-A and vit-C on the in vitro expression pattern of milk-specific proteins in MAC-T cells was investigated by quantitative RT-PCR. The results showed that the efficacies of stabilized nanoemulsions of vit-A and vit-C were 100% and 92.7%, respectively. The particle sizes were around 475.7 and 225.4 nm, and the zeta potentials were around -33.5 and -21.3 mV, respectively. The expression changes of alpha(s2)-, beta- and kappa-casein were higher in the presence of a stabilized nanoemulsion of vit-A, compared with nanoemulsion-free vit-A. Furthermore, the expression changes of alpha(s2)- and beta-casein were lower and that of kappa-casein was higher in the presence of a stabilized nanoemulsion of vit-C, compared with nanoemulsion-free vit-C. Thus, our findings demonstrate the efficacy of nanoemulsified vit-A and vit-C in changing the expression of milk-specific proteins in MAC-T cells.
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Collections - Graduate School > Department of Food and Biotechnology > 1. Journal Articles
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