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Enzymatic Preparation of Maltooctaose-rich Mixture from Starch Using a Debranching Enzyme of Nostoc punctiforme

Authors
Choi, Ji-HyeKim, Myo-JeongKim, Young-WanLee, HeeseobPark, Jong-TaeLee, Byong-HoonPark, Kwan-Hwa
Issue Date
Apr-2009
Publisher
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
Keywords
Nostoc punctiforme; cyanobacteria; debranching enzyme; maltooctaose-rich mixture; maltooligosaccharide
Citation
FOOD SCIENCE AND BIOTECHNOLOGY, v.18, no.2, pp.570 - 573
Indexed
SCIE
SCOPUS
KCI
Journal Title
FOOD SCIENCE AND BIOTECHNOLOGY
Volume
18
Number
2
Start Page
570
End Page
573
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/120331
ISSN
1226-7708
Abstract
The debranching enzyme of Nostoc punctiforme (NPDE) is a novel enzyme that catalyzes the hydrolysis of alpha-1,6-glycosidic linkages in starch, followed by the sequential hydrolysis of alpha-1,4-glycosidic linkages. The debranching activity of NPDE is highly specific for branched chains with a degree of polymerization (DP)>8. Moreover, the rate of hydrolysis of alpha-1,4-linkages by NPDE is greatly enhanced for maltooligosaccharides (MoS) With a DP>8. An analysis of reaction mixtures containing various starches revealed the accumulation of maltooctaose (G8) with glucose and maltose. Based on the novel enzymatic properties of NPDE, an MO mixture containing more than 60% G8 with yield of 18 g G8 for 100 g starch was prepared by the reaction of NPDE with soluble starch, followed by ethanol precipitation and gel permeation chromatography (GPC). The yield of the G8-rich mixture was significantly improved by the addition of isoamylase. In summary, a 4-step process of the production of a G8-rich mixture was developed involving the enzymatic hydrolysis of starch by NPDE.
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