Beneficial effect of the unsaponifiable matter from rice bran on oxidative stress in vitro compared with alpha-tocopherol
- Authors
- Lee, JW; Lee, SW; Kim, MK; Rhee, C; Kim, IH; Lee, KW
- Issue Date
- Feb-2005
- Publisher
- WILEY
- Keywords
- gamma-oryzanol; oxidative stress; rice bran; supercritical CO2; tocopherol; unsaponifiable matter
- Citation
- JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, v.85, no.3, pp.493 - 498
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
- Volume
- 85
- Number
- 3
- Start Page
- 493
- End Page
- 498
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/123260
- DOI
- 10.1002/jsfa.2009
- ISSN
- 0022-5142
- Abstract
- Unsaponifiable matter (UM) was prepared from rice bran using n-hexane extraction followed by removal of its fatty acid methyl ester with supercritical CO2 under heat-stable conditions. The UM was made up of 1% of vitamin E isomers, 28% of gamma-oryzanol and 71% of uncharacterized compounds. The aim of this study was to determine the antioxidant activities of the UM, using alpha-tocopherol (alpha-T) as a positive control, by measuring the Fe3+-reducing antioxidant power (FRAP), 2,2-diphenyl-l-picrylhydrazyl (DPPH.) free-radical-scavenging property and lipid peroxidation in rat liver microsomes. In addition, the effects of the UM on the tert-butyl hydroperoxide (t-BOOH)-induced cytotoxicity in cultured rat hepatocytes were also investigated. In FRAP assay and DPPH. free-radical-scavenging assay, the results were expressed g(-1) alpha-T or g(-1) UM. The amount of UM used in lipid peroxidation assay and cytotoxicity assay was the amount required to have equal amounts of total vitamin E isomers in the sample and the control alpha-T. The UM, as well as alpha-T, exhibited significant antioxidant activities in FRAP, radical-scavenging and membrane-lipid oxidation. The FRAP value for total vitamin E isomers in the UM (TVEIUM) was 9.1 times higher than that for alpha-T. In terms of their capacities to perform radical-scavenging and lipid peroxidation, both TVEIUM and alpha-T showed similar antioxidant activities. In experiments using cultured rat hepatocytes, the t-BOOH-induced lactate dehydrogenase release was significantly inhibited by the addition of 63.5 and 160 mug ml(-1) of TVEIUM treatments (84 and 89%, respectively), and that of 63.5 and 160 mug ml(-1) of alpha-T treatment (88 and 93%, respectively). The antioxidant function against oxidative stress of the UM prepared from rice bran may extend its use to being a potential dietary supplement. (C) 2004 Society of Chemical Industry.
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Collections - College of Life Sciences and Biotechnology > Division of Food Bioscience and Technology > 1. Journal Articles
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