Comparison of ELISA and HPLC methods for the determination of biogenic amines in commercial doenjang and gochujang
- Authors
- Kim, Tae-Kyun; Lee, Jae-Ick; Kim, Jin-Hyo; Mah, Jae-Hyung; Hwang, Han-Joon; Kim, Young-Wan
- Issue Date
- 12월-2011
- Publisher
- SPRINGER
- Keywords
- biogenic amine; doenjang; gochujang; competitive direct enzyme-linked immunosorbent assay; HPLC
- Citation
- FOOD SCIENCE AND BIOTECHNOLOGY, v.20, no.6, pp.1747 - 1750
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- FOOD SCIENCE AND BIOTECHNOLOGY
- Volume
- 20
- Number
- 6
- Start Page
- 1747
- End Page
- 1750
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/110996
- DOI
- 10.1007/s10068-011-0241-0
- ISSN
- 1226-7708
- Abstract
- In order to conduct fast detection of biogenic amines (BAs) in doenjang and gochujang, a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for histamine was compared with HPLC analysis. The histamine contents were determined from 0.7 to 420 mg/kg by CD-ELISA and 2.2 to 382.4 mg/kg by HPLC. Both methods showed a good agreement for histamine analysis (R=0.969). In addition, the histamine contents by CD-ELISA were correlated to the sum of 7 major BAs in the samples (R=0.910), suggesting that the CD-ELISA can be used as a rapid indicator for total BAs in doenjang and gochujang.
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Collections - Graduate School > Department of Food and Biotechnology > 1. Journal Articles
- College of Science and Technology > Department of Food and Biotechnology > 1. Journal Articles
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