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Comparison of ELISA and HPLC methods for the determination of biogenic amines in commercial doenjang and gochujang

Authors
Kim, Tae-KyunLee, Jae-IckKim, Jin-HyoMah, Jae-HyungHwang, Han-JoonKim, Young-Wan
Issue Date
12월-2011
Publisher
SPRINGER
Keywords
biogenic amine; doenjang; gochujang; competitive direct enzyme-linked immunosorbent assay; HPLC
Citation
FOOD SCIENCE AND BIOTECHNOLOGY, v.20, no.6, pp.1747 - 1750
Indexed
SCIE
SCOPUS
KCI
Journal Title
FOOD SCIENCE AND BIOTECHNOLOGY
Volume
20
Number
6
Start Page
1747
End Page
1750
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/110996
DOI
10.1007/s10068-011-0241-0
ISSN
1226-7708
Abstract
In order to conduct fast detection of biogenic amines (BAs) in doenjang and gochujang, a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for histamine was compared with HPLC analysis. The histamine contents were determined from 0.7 to 420 mg/kg by CD-ELISA and 2.2 to 382.4 mg/kg by HPLC. Both methods showed a good agreement for histamine analysis (R=0.969). In addition, the histamine contents by CD-ELISA were correlated to the sum of 7 major BAs in the samples (R=0.910), suggesting that the CD-ELISA can be used as a rapid indicator for total BAs in doenjang and gochujang.
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Graduate School > Department of Food and Biotechnology > 1. Journal Articles
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